Capto plasmid select6/23/2023 Magnesium sulfate and potassium phosphates are added in order to provide a magnesium, sulfur, potassium and a phosphorous source (the phosphates also function as buffering agent). Nitrogen is typically supplied by adding complex components such as yeast extract, casamino acids, or peptones. Glycerol can also aid in supporting reduced maximum specific growth rates. This can be avoided by (partially) substituting glucose for glycerol. However, glucose may cause over production of acetate (Crabtree effect). yeast extract) supply growth factors, amino acids, purines and pyrimidines.Īs carbon source, glucose is conventionally used as it is efficiently metabolized and inexpensive. On the other hand, semi-defined media can support higher cell densities as the complex components (e.g. With minimal media, highly reproducible batches can be obtained. Both minimal and semi-defined media can be used. The general media composition consists of a carbon source, a nitrogen source, magnesium sulfate, di- and monopotassium phosphate, and minerals. Several parameters can affect the bacterial productivity like the master cell bank selection, the growth rate, the culture medium, the feeding rate as well as the appropriate growth conditions and parameters like pH, osmolarity, optical density (DO) and temperature. Laurens holds a Masters Degree in Industrial Biochemical Engineering from the University of Leuven (Belgium) and his main passion is sharing knowledge. Being able to work with different kinds of manufactures, Laurens Vergauwen has gained a strong knowledge about purification strategies for different kinds of biopharmaceuticals, including plasmid DNA. Laurens is a downstream processing expert supporting customers in the development and optimization of various downstream purification technologies (chromatography, TFF, clarification, sterile and viral filtration). Laurens Vergauwen, Process Development Scientist Nargisse El Hajjami is focusing on cell and gene therapy business development by leading strategic initiatives, supporting go-to-market strategies, and supporting customers to build, grow and optimize their knowledge and manufacturing processes. Molecular microbiologist and bioprocess expert with 10 years’ experience combining scientific research, process development, and biomanufacturing engineering. Nargisse El-Hajjami, PhD, Associate Director, EMEA Segment Development for Cell and Gene Therapy For this Ask the Expert Session, we assembled a team of experts to answer questions on plasmid DNA production for cell and gene therapy applications. With the high demand and quality requirements, it is important to seek out experts in the field who can illuminate the critical optimization opportunities for pDNA and answer questions about the production. Thus, understanding all areas of the process are critical to successful manufacturing at a large scale. Plasmid DNA (pDNA) manufacturing faces several challenges due to its large size, shear sensitivity, high viscosity, and the similarities between the pDNA and the impurities present during manufacturing. This has led to the need to optimize manufacturing to meet the demand for volume as well as the quality required for use in the manufacture of therapeutics. High-quality plasmid DNA is a key component in cell and gene therapy manufacturing and as such is in high demand. Job Title: Associate Director, EMEA Segment Development for Cell and Gene Therapy
0 Comments
Leave a Reply. |